21 articles, 3 reviews, 1 book chapter.


Crystal structure of the thioesterase SgcE10 supporting commonpolyene intermediates in 9- and 10-membered enediyne core biosynthesis

Annaval, T., Rudolf, J. D.; Chang, C.-Y.; Lohman, J. R.; Kim, Y.; Bigelow, L.; Jedrzejczak, R.; Babnigg, G.; Joachimiak, A.; Phillips Jr., G. N.; Shen, B. ACS Omega 2017, 2, 5159–5169.

Cytochromes P450 for natural product biosynthesis in Streptomyces: sequence, structure, and function

Rudolf, J. D.;* Chang, C.-Y.;* Ma, M.; Shen, B. Nat. Prod. Rep. 2017, 34, 1141–1172.

The role of combinatorial biosynthesis in natural products discovery

Rudolf, J. D.;* Crnovcic, I.;* Shen, B. Chapter 3 in Chemical Biology of Natural Products. Newman, D. J.; Cragg, G. M.; Grothaus, P.G., Eds. CRC Press, 2017, pp. 87–125, in press.

Nutritional control of antibiotic production by Streptomyces platensis MA7327: importance of L-aspartic acid

Falzone, M.; Crespo, E.; Jones, K.; Khan, G.; Korn, V.; Patel, A.; Patel, M.; Patel, K.; Perkins, C.; Siddiqui, S.; Stenger, A.; Yu, E.; Gelber, M.; Scheffler, R.; Nayda, V.; Ravin, A.; Komal, R.; Rudolf, J. D.; Shen, B.; Gullo, V.; Demain, A. J. Antibiot2017, 70, 828–831.

Platensimycin and platencin: inspirations for chemistry, biology, enzymology, and medicine 

Rudolf, J. D.; Dong, L.-B.; Shen, B. Biochem. Pharm2017, 133, 139–151.

Germicidins H–J from Streptomyces sp. CB00361 

Ma, M.; Rateb, M. E.; Yang, D.; Rudolf, J. D.; Zhu, X.; Huang, Y.; Zhao, L.-X.; Jiang, Y.; Duan, Y.; Shen, B. J. Antibiot. 2017, 70, 200–203.

In vivo instability of platensimycin and platencin: synthesis and biological evaluation of urea- and carbamate-platensimycin 

Dong, L.-D.;* Rudolf, J. D.;* Lin, L.; Ruiz, C.; Cameron, M. D.; Shen, B. Bioorg. Med. Chem. 2017, 25, 1990–1996.


Biosynthetic origin of the ether ring in platensimycin 

Rudolf, J. D.;* Dong, L.-D.;* Manoogian, K.; Shen, B. J. Am. Chem. Soc. 2016, 138, 16711–16721.

Strain prioritization and genome mining for enediyne natural products

Yan, X.;* Ge, H.-M.;* Huang, T.;* Hindra;* Yang, D.; Teng, Q; Crnovcic, I.; Li, X.; Rudolf, J. D.; Lohman, J. R.; Gansemans, Y.; Zhu, X.; Huang, Y.; Zhao, L.-X.; Jiang, Y.; van Nieuwerburgh, F.; Rader, C.; Duan, Y.; Shen, B. mBio 2016, 7, e02104–16.

Highlighted in news reports including TSRI News and ADC Review.

A mutasynthetic library of platensimycin and platencin analogues

Dong, L.-B.;* Rudolf, J. D.;* Shen, B. Org. Lett. 2016, 18, 4606–4609.

Crystal structures of SgcE6 and SgcC, the two-component monooxygenase that catalyzes hydroxylation of a carrier protein-tethered substrate during the biosynthesis of the enediyne antitumor antibiotic C-1027 in Streptomyces globisporus

Chang, C.-Y.; Lohman, J. R.; Cao, H.; Tan, K.; Rudolf, J. D.; Ma, M.; Xu, W.; Bingm, C. A.; Yennamalli, R. M.; Bigelow, L.; Babnigg, G.; Yan, X.; Joachimiak, A.; Phillips Jr., G. N.; Shen, B. Biochemistry 2016, 55, 5142–5154.

Structure of the ent-copalyl diphosphate synthase PtmT2 from Streptomyces platensis CB00739, a bacterial type II diterpene synthase

Rudolf, J. D.;* Dong, L.-B.;* Cao, H.; Hatzos-Skintges, C.; Osipiuk, J.; Endres, M.; Chang, C.-Y.; Ma, M.; Babnigg, G.; Joachimiak, A.; Phillips Jr., G. N.; Shen, B. J. Am. Chem. Soc. 2016, 138, 10915–10915.

Highlighted in news reports including TSRI News, American Laboratory, and Science Newsline.

Crystal structure of SgcJ, an NTF2-like superfamily protein involved in biosynthesis of the nine-membered enediyne antitumor antibiotic C-1027

Huang, T.; Chang, C.-Y.; Lohman, J. R.; Rudolf, J. D.; Kim, Y.; Chang, C.; Yang, D.; Ma, M.; Yan, X.; Crnovcic, I.; Bigelow, L.; Clancy, S.; Bingman, C. A.; Yennamalli, R. M.; Babnigg, G.; Joachimiak, A.; Phillips Jr., G. N.; Shen, B. J. Antibiot. 2016, 69, 731–740.

Antibacterial sulfur-containing platensimycin and platencin congeners from Streptomyces platensis SB12029

Dong, L.-B.;* Rudolf, J. D.;* Shen, B. Bioorg. Med. Chem. 2016, 24, 6348–6353.

Genome neighborhood network reveals insights into enediyne biosynthesis and facilitates prediction and prioritization for discovery 

Rudolf, J. D.;* Yan, X.;* Shen, B. J. Ind. Microbiol. Biotechnol. 2016, 43, 261–276.


Crystal structure of the zorbamycin-binding protein ZbmA, the primary self-resistance element in Streptomyces flavoviridis ATCC21892  

Rudolf, J. D.; Bigelow, L.; Chang, C.; Cuff, M. E.; Lohman, J. R.; Chang, C.-Y.; Ma, M.; Yang, D.; Clancy, S.; Babnigg, G.; Joachimiak, A.; Phillips Jr., G. N.; Shen, B. Biochemistry 2015, 54, 6842–6851.

Angucyclines and angucyclinones from Streptomyces sp. CB01913 featuring C-ring cleavage and expansion  

Ma, M.; Rateb, M., Teng, Q., Yang, D., Rudolf, J. D.; Zhu, X.; Huang, Y.; Zhao, L.-X.; Jiang, Y.; Li, X.; Rader, C.; Duan, Y.; Shen, B. J. Nat. Prod. 2015, 78, 2471–2480.

A genetically amenable platensimycin- and platencin-overproducer as a platform for biosynthetic explorations: a showcase of PtmO4, a long-chain acyl-CoA dehydrogenase 

Rudolf, J. D.;* Dong, L.-B.; Huang, T.; Shen, B. Mol. BioSyst. 2015, 11, 2717–2726.

Highlighted as a 2015 Hot Article in Mol. BioSyst.10th Anniversary of Mol. BioSyst. cover article.

Enediynes: exploration of microbial genomics to discover new anticancer drug leads  

Shen, B.; Hindra; Yan, X.; Huang, T.; Ge, H.-M.; Yang, D.; Qihui, T.; Rudolf, J. D.; Lohman, J. R. Bioorg. Med. Chem. Lett. 2015, 25, 9–15.


BlmB and TlmB provide resistance to the bleomycin family of antitumor antibiotics by N-acetylating metal-free bleomycin, tallysomycin, phleomycin, and zorbamycin  

Coughlin, J. M.;* Rudolf, J. D.;* Wang, L.; Galm, U.; Wendt-Pienkowsky, E.; Tao, M.; Shen, B. Biochemistry 2014, 53, 6901–6909.

Strain prioritization for natural product discovery by a high-throughput real-time PCR method 

Hindra;* Huang, T.;* Yang, D.;* Rudolf, J. D.;* Xie, P.; Xie, G.; Teng, Q.; Lohman, J. R.; Zhu, X.; Huang, Y.; Zhao, L.-X.; Jiang, Y.; Duan, Y.; Shen, B. J. Nat. Prod. 2014, 77, 2296–2303.

Highlighted in C&E News, Oct. 6, 2014.

Enediyne polyketide synthases stereospecifically reduce the β-ketoacyl intermediates to β-D-hydroxyacyl intermediates in enediyne core biosynthesis 

Ge, H.-M.; Huang, T.; Rudolf, J. D.; Lohman, J. R.; Huang, S.-X.; Guo, X.; Shen, B. Org. Lett. 2014, 16, 3958–3961.

Biosynthetic potential-based strain prioritization for natural product discovery – a showcase for diterpenoid producing actinomycetes 

Xie, P.;* Ma, M.;* Rateb, M.; Shaaban, K.; Yu, Z.; Huang, S.-X.; Zhao, L.-X.; Zhu, X.; Yan, Y.; Peterson, R. M.; Lohman, J. R.; Yan, D.; Yin, M.; Rudolf, J. D.; Jiang, Y.; Duan, Y.; Shen, B. J. Nat. Prod. 2014, 77, 377–387.

Mechanisms of self-resistance in the platensimycin and platencin producing Streptomyces platensis MA7327 and MA7339 strains  

Peterson, R. M.;* Huang, T.;* Rudolf, J. D.; Smanski, M. J.; Shen, B. Chem. Biol. 2014, 21, 389–397.

Highlighted in news reports including TSRI News, The Scientist Magazine, and Genetic Eng. & Biotech. News.


Tyrosine O-prenyltransferase SirD catalyzes S-, C- and N-prenylations on tyrosine and tryptophan derivatives  

Rudolf, J. D.; Poulter, C. D. ACS Chem. Biol. 2013, 8, 2707–2714.

Multisite prenylation of 4-substituted tryptophans by dimethylallyltryptophan synthase

Rudolf, J. D.; Wang, H.; Poulter, C. D. J. Am. Chem. Soc. 2013, 135, 1895–1902.